(GIST OF SCIENCE REPORTER) MCQs on Protoplasts

(MARCH-2024)

MCQs on Protoplasts

1.   Protoplasts are plant cells with a plasma membrane but no _________.

1. Cell wall
2. Ribosome
3. Mitochondria
4. None of the above

2.   Protoplast is counted by _________.

1. Hemocytometer
2. Protocytometer
3. Gigermuller counter
4. None

3. Intact protoplasts from a pellet. This statement is_________.

1. False
2. True
3. Not known
4. None

4.    Protoplast is a _________ individual cell.

1. Functional
2. Non Functional
3. Not Known
4. None

5.   The word protoplast was coined by _________.

1. Hanstein
2. Klercker
3. Cocking
4. Tkebe et al

6.  To isolate protoplast, one needs _________.

1. Pectinase
2. Cellulase
3. Both  Pectinase & Cellulase
4. Chitinase

7.   Which of the following is correct about Cybrid?

1. Genomes of two different plants
2. Cytoplasms of two different plants
3. Genomes and Cytoplasms of two different plants
4. Cytoplasms of two different plants & the genome of one plant

8. The contact of the protoplast depends on the surface _________.

1. Charge                 
2. Permeability 
3. Heat                      
4. None 

9. Which of the following scientists tried to isolate protoplast from plasmolysed higher plant cells by mechanically cutting the cells?

1. Takabe                               
2. Klercker 
3. Carlson                               
4. None 

10. _________ is used to check the viability of the protoplast.

1. Phenosafranine staining       
2. Fluorescein Diacetate (FDA) staining        
3. Both “a” and “b”                   
4. Only “a”

11.  The first intergeneric hybrid (Pomato) between Potato and Tomato was demonstrated by _________.       

1. Melchers
2. Zelcer
3. Carlson
4. Takabe

12.  The fusion and fusion product of protoplast is observed in _________.

1. Electron Microscope
2. Compound Microscope
3. Phase Contrast Microscope
4. Inverted Microscope

13.   There are several chemical and physical agents known as fusogens which help in fusion between protoplasts.  These are _________.

1. Sodium Nitrate            
2. Polyethylene Glycol
3. Dextran Sulphate
4. All

14.   In some species, protoplasts can directly form somatic embryos, These are _________.

1. Medicago
2. Brassica juncea   
3. Citrus
4. All

15. Somatic hybrids are produced by _________.

1. Protoplast fusion
2. Tissue culture  
3. Pollen culture 
4. Hybridoma process

16. The enzyme used for the isolation of protoplast is _________.

1. Amylase 
2. Cellulase 
3. Catalase 
4. All

17. The most suitable source for protoplast isolation in plants is _________.

1. Leaf
2. Stem tip
3. Lateral meristem 
4. Apical meristem

18. For culturing Protoplast, which media is generally used?

1. Natural media              
2. Nutritional media  
3. Differential media
4. Artificial media

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Courtesy: Science Reporter